원문정보
초록
영어
Para-nitrobenzyl alcohol (pNB-OH) is used to protect carboxylic acid functionalities in synthetic process of cephalosporin-derived antibiotics. Deprotection procedure usually involves environmental toxicant such as catalytic zinc in organic solvents. This process generates large amounts of solvent and zinc-containing waste material. With the increased awareness for environmentally-friendly processes and more cost-effective alternatives, reaserchers began searching for an esterase for the deprotection reaction. Previous studies reported para-nitrobenzyl esterase (PNBE) from Bacillus subtilis that catalyze the hydrolysis of the p-nitrobenzyl esters of various cephalosporin derived antibiotics. Although the reaction yield was high, the enzyme preparation could not complete with the economy. In this study, the para-nitrobenzyl esterase (PNBE) was successfully immobilized onto electrospun polystyrene/poly(styrene-co-maleic anhydride) (PS/PSMA) nanofibers as cross-linked enzyme aggregates (CLEA). The maximum amount of PNBE immobilized on the PS-PSMA nanofibers was determined to be 56.4 mg/g of nanofibers. Compared to its free form, immobilized PNBE-CLEA exhibited improved stability in various areas such as temperature and storage. These results demonstrate that PNBE-CLEA immobilized nanofiber could be used as an economical and environmentally-friendly system for the catalytic process of para-nitrobenzyl esters in pharmaceutical synthesis.