earticle

영어

Enzyme catalyzed hydrolysis of hemicellulose in biomass feedstocks require multiple enzymes to achieve saccharification to fermentable monosaccharides. The minimal enzyme requirement in the cocktail for maximum saccharification can be achieved by using statistical experimental design. In the present study Response surface methodology was used to optimize maximum saccharification of water soluble wheat arabinoxylan and subsequently the Korean barley straw by employing an enzyme cocktail consisting of recombinanat xylanase, arabinanase and β- xylosidase/L-arabinofuranosidase of Phanerochate chrysosporium. A central composite design (CCD) was used to evaluate and confirm the effectiveness and interactions of the above enzymes along with time (h). A maximum saccharification of 100% was achieved when one gram water soluble wheat arabinoxylan was used as substrate wherein the requirement of recombinanant xylanase, arabinanase, xylosidase/ arabinofuranosidase and time were 400 U, 150 U, 3000 U and 40 min, respectively. Subsequently when pretreated Korean barley straw was used as a substrate, a maximum saccharification of 42% was achieved by using an enzyme cocktail of 25 U xylanase, 5 U arabinanase and 200 U xylosidase/arabinofuranosidase with a time period of 12 h on 1 gram substrate. The experimental values under the optimum conditions agreed well with model predictions and the effectiveness of the synergetic action of the enzymes involved in the minimal cocktail.