원문정보
초록
영어
Proteases hydrolyze the peptide bond of proteins by recognizing the specific amino acid sequences and are involved in various biological processes via the cleavage reaction. There has been a vision of developing the proteases as therapeutics by engineering them to target disease-causing proteins, which can be achieved by engineering the specificities and activities of the enzymes. We intend to apply the directed evolution approach to engineer a protease in the apoptosis pathway, caspase-3, to inactivate TNF-alpha that is known to be associated with autoimmune diseases. In this presentation, we will describe our current effort to develop a high-throughput method based on fluorescence signal to screen caspase-3 libraries, which can be applied in general for protease engineering.