원문정보
초록
영어
Long-chain ω-hydroxy fatty acids are widely being used in many industrial fields due to its two functional groups, unlike other natural fatty acids. Chemical synthesis of ω-hydroxy fatty acids from monocarboxylic acid, palmitic acid for example, however, is not appropriate since the primary carbon has less reactivity than the secondary carbon. Currently, the production of ω-hydroxy fatty acids relies on biotransformation by Candida tropicalis. The problems with this method are that eukaryotes are not able to grow and produce desired compounds fast, and C.tropicalis ultimately oxidizes ω-hydroxy fatty acids to α,ω-dicarboxylic acids. Therefore, we expressed CYP153A from Marinobacter aquaeolei, CamA, and CamB in E. coli BL21(DE3) to overcome existing limitation. Through resting cell reaction in shaking flask, 1 mM of hexadecanoic acid was converted to 16-hydroxyhexadecanoic acid with 90.4 % yield in 6 hours. Biphasic reaction was then performed to extract the product simultaneously and increase initial substrate concentration. Various solvents were screened in terms of log POW, partition coefficient of the product, and productivity.