원문정보
초록
영어
Klebsiella oxytoca is a promising microorganism for 2,3-butanediol (2,3-BD) production. 2,3-BD can be used as a platform chemical intended for the production of synthetic rubbers, plastics, and solvents. However, K. oxytoca wild type strain produced a significant amount of lactic acid along with 2,3-BD. Thus, a mutant was constructed by disrupting theldhA gene which encodes lactate dehydrogenase to reduce lactic acid formation. The mutant resulted in an increase of 54%, 13%, 60%, and 78% of 2,3-BD titer, productivity, yield, and selectivity, respectively, compared with the wild strain. A fed-batch fermentation by this mutant with intermittent glucose feeding produced115 g/L of 2,3-BD with an yield and productivity of 0.41 g/g glucose and 2.27 g/L/h.