earticle

영어

As completely biodegradable polyesters, polyhydroxyalkanoates (PHAs) are synthesized by various bacterial strains. Usually PHAs are synthesized under stress conditions as organic carbon. Ralstonia eutropha H16 (KCTC 22469) is a famous lithoautotrophic bacterium. R. eutropha H16 can use both organic compounds and H2 as its energy sources and it has ability of storing PHAs. Since many studies for increasing products using electrochemical bioreactors were reported, increasing production of PHAs could be expected when R. eutropha H16 could use electron from electrode. Electricity is considered an energy source because R. eutropha H16 uses H2 and CO2 as its energy and carbon sources, respectively. Consuming of H2 and CO2 was observed in R. eutropha H16 when headspace was made up of H2, CO2, and O2. Instead of H2, electron from electrode could be energy source. R. eutropha H16 was cultured in H-type Bio-Electro-Reactor (H-BER) and serum bottle, respectively. As control experiments, R. eutropha H16 was grown under autotrophic condition at 30℃ in Basal Mineral Medium with Ar : CO2 : O2 = 8 : 1 : 1 and H2 : CO2 : O2 = 8 : 1 : 1 as the gas phase respectively. Dry cell weight of R. eutropha H16 was measured for the comparison between H-BER and serum bottle. As a result, R. eutropha H16 was grown under supplying CO2 and electron as a carbon and an energy source, respectively.