earticle

영어

Niram was used for dyeing of cotton and fabrics as traditional indigo dye. In order to dye on cotton, Niram must divert from insoluble indigo to water-soluble leuco-indigo by reduction reaction. In this study, microorganisms in Niram were involved in a reduction reaction during fermentation of Niram. Microorganisms in traditional Niram were isolated and identified. The phylogenetic analysis revealed that strain Niram A and B were closely related to the members of the genus Comamonas and Microbacterium, respectively. Strain Niram A exhibited the highest 16S rRNA gene sequence similarity to C. aquatica LMG 2370T (98.06%). Strain Niram B showed 100% homology with M. oxydans DSM 20578T and M. maritypicum DSM 12512T. The growth of the strain Niram A and B was not inhibited in Niram medium containing high calcium concentration without free sugar as carbon source. To analyze the reducing ability of isolated microorganisms, the color change of Niram medium fermented for 15 days (100 rpm, pH 10, and 30°C) was analyzed by a color-difference meter. In all fermented samples for 10 days, the a* values showed no significant difference. In fermented samples for 15days, the control had -3.96±0.02 of the a* value. On the other hand, the Niram samples fermented with the strain Niram A and B showed -4.20±0.02 of the a* value and -7.86±0.03 of the a* value, respectively. In the reducing ability on the Niram, strain Niram B was significantly better than strain Niram A.