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The sophorolipids are surface-active compounds have been known for over 50 years, but because of growing awareness in herbal medicine and cosmetics, they recently regained the attention. Rice bran is a byproduct of the rice milling process. By Composition 12-13% of oil content was reported in rice bran along a high level of dietary fibers, and beneficial metabolites. These metabolites were reported as a stimulant for the dermal fibroblast proliferation and collagen neosynthesis by its macrophage-activating and fibrinolytic properties. They also act as an inhibitor to free radical and elastase activity, as desquamating and depigmenting agents. Because of small chain size of the oil present in rice bran, it was considered to be hypoallergenic and easier penetrative to skin barrier. This study was mainly designed to produce and optimize the sophorolipid using rice bran as a substrate. The implementation of operational conditions of fermentation was done with 30L industrial fermenter, using Candida bombicola ATCC-22214 as producing organism. Media engineering was done with different combinations substrates mainly rice bran, glucose and rapeseed oil. The broth was extracted in alcohol followed by n-Hexane extraction to achieve partially purified sophorolipid. The sophorolipids produced by different combination of media constituents were subjected to screen the surface properties like surface tension, CMC, minimum S.T., dispersion power, foaming power and detergency, and bioactive properties like antimicrobial activity, cytotoxicity, effect on melanogenesis. The results were found to be encouraging in regard to bioactivities as well as production cost effectiveness.