원문정보
초록
영어
Current commercial 1,3-Propanediol(1,3-PD) production is based on chemical production. This process requires harmful chemical solvents, high-temperature, high pressure conditions and expensive catalysts. So much attention has been paid to biosynthesis because it uses usually renewable feedstock and does not generate harmful by-products compare to chemical production. The dha regulon in Clostridium butyricum enables the organism to grow anaerobically on glycerol and produce 1,3-propanediol (1,3-PD). Escherichia coli, which does not have a dha system, is unable to grow anaerobically on glycerol without an exogenous electron acceptor and does not produce 1,3-PD. So we had cloning dhaB1,2 and yqhD genes into pQE30 vector, and made 1,3-PD synthesis recombinant E.coli XL1-blue. DhaB1 and dhaB2 genes each encoded glycerol dehydratase (GDHt) and GDHt reactivator from Clostridium butyricum KCTC3700. And yqhD gene encoded 1,3-PD oxido-reductase isoenzyme from escherichia coli JM109. The recombinant E. coli XL1 blue was cultured under several conditions to find out the optimal condition for 1,3-PD production. Further improvement of the fermentation by K.pneumoniae and E.coli should be focused on increasing the product tolerance of the strain and reducing the ethanol formation.