earticle

영어

3-Hydroxypropionic acid (3-HP) is a valuable platform chemical used in the production of several industrially important chemicals. Biologically, 3-HP can be produced from glycerol by two enzymatic reactions catalyzed by glycerol dehydratase (dhaB) and aldehyde dehydrogenase (aldH). Glycerol dehydratase, a coenzyme B12 dependent enzyme is a rate limiting step in the production of 3-HP. The second enzyme aldehyde dehydrogenase requires continuous supply of NAD+ for uninterreputed production of 3-HP, which can be efficiently produced in the presence of oxygen via oxidative phosphorylation. Pseudomonas denitrificans, a Gram-negative facultative anaerobic bacterium is known to produce coenzyme B12 in the presence of oxygen and considered as a promising host organism for the biological production of 3-HP. In this study, to have better understanding of this microorganism the complete genome was sequenced and annoated. The genome analysis showed the presence of genes for coenzyme B12 biosynthsis, pollutant degradation, putative aldehyde dehydrogenases and oxidoreductases. It also showed that this microorganism lack genes for glycerol facilitator (glpF), 6-phosphofructokinase(pfk) an enzyme of Embden–Meyerhof parnas (EMP) pathway and phosphogluconate dehydrogenase (gnd) involved Entner–Doudoroff (ED) pathway indicating inefficiency to utilize various carbon substrates such as glycerol and glucose. This study also describes the strategies to develop efficient recombinant strain to produce 3-HP from glycerol.