earticle

영어

Since the late 1990s, magnetoelectronics has attracted a great deal of attention for biosensor development because magnetoresistive (MR) detection using magnetic particles has shown high sensitivity, cheap device, and easy transfer of magnetic particles by magnetic field.1 Iron oxide-based magnetic nanoparticles (MNPs) enable high-density binding across the sensor surface.2 However, these metal oxide nanoparticles tend to easily form aggregates due to magnetic dipolar interaction between adjacent MNPs.3 Thus, the surface modification of MNPs has been an important issue to prevent aggregation and to endow functional groups for conjugation with biomolecules such as proteins, enzymes, and DNA, through covalent bonds. In this work, we report the direct surface modification of commercially available γ-Fe2O3 MNPs with bifunctional organic molecules. After surface modification of MNPs, the prove oligonucleotide with a specific sequence immobilizes on the MNPs and hybridizes with complementary/non-complementary target oligonucleotide. After hybridization of target oligonucleotide, the detection of specific binding with complementary oligonucleotide is evaluated employing a confocal microscopy.