earticle

영어

An optical biosensor for the osteoarthritis (OA) diagnosis based on the quantification of OA biomarker in human serum and urine was developed. In the OA progresses, the substances of joint such as cartilage protein are degraded and secreted into serum and urine. These cartilage degradation proteins such as C-telopeptide of type II collagen (CTX-II) are used as significant OA biomarker. The CTX-II is classified as serum CTX-II (sCTX-II) and urine CTX-II (uCTX-II) according to its existence site. Due to the structural differences between sCTX-II (homo-dimer) and uCTX-II (monomer), two different types of immunoassays such as competitive immunoassay for uCTX-II and sandwich assay for sCTX-II are employed. For the simultaneous quantification analysis of these markers, we developed a fluoromicrobeadbased multiplex guiding chip [1]. On the chip interface, CTX-IIs are bio-specifically captured and its aspect is appeared as formation of fluorescent dot. By counting the number of bead on sensing surfaces, the concentrations of CTX-II were quantified. The obtained optical signal showed good correlation with sCTX-II concentration from 6.3 to 250 pg/ml and uCTX-II concentration from 1.4 to 10 ng/ml.