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영어

This research describe a new monitoring tool capable of concurrently evaluation of two kinds of chemical: reactive oxygen species (ROS) and non-oxidative stress-making (NOSM) compound based on lysosomal response in the Saccharomyces cerevisiae. Exposure of some organisms to oxidative stresses due to toxins alters lysosomal enzymes, thus we used intracellular lysosomes in S. cerevisiae as a biomonitoring tool to detect oxidative stresses by pesticides (glyphosate and methidathion) and heavy metals (sodium meta-arsenite and cadmium nitrate). Beside this, the effects of tetracycline and aspirin were evaluated simultaneously to examine NOSM compounds influence. The lysosomes ability to detect toxicity was evaluated by using confocal microscopy after staining lysosomes with LysoTracker. Treatment of S. cerevisiae with toxins increased the number of conspicuous and red lysosome-like organelles surrounding S. cerevisiae nucleic. The ROS levels were determined synchronously by staining the yeast cells with carboxy-H2- dichloro-dihydrofluorescein diacetate (carboxy-H2-DCFDA). The results indicated that each chemical has an optimal concentration at which the quantity of lysosomes reach the peak while the growth of yeast were not affected. It means that our method can detect the sublethal concentrations which do not inhibit the cells growth of these chemicals.