원문정보
초록
영어
The seaweed Gelidium amansii was fermented to produce bioethanol after thermal acid hydrolysis pretreatment and enzymatic saccharification. Optimal pretreatment conditions of 94 mM H2SO4 and 10% (w/v) seaweed slurry at 121°C for 60 min were determined and mono sugars of 43.5 g/L with 60.7% of theoretical yield were obtained by enzymatic saccharification of the seaweed slurry after thermal acid hydrolysis. G. amansii hydrolysate was used as biomass for ethanol production by separate hydrolysis and fermentation (SHF), and the ethanol yield of 20.2 g/L was obtained by Pichia stipitis KCTC 7228. HPLC of batch fermentations showed that 5-hydroxymethylfurfural (HMF) was converted to 2,5-bis-hydroxymethylfuran. The accumulated 2,5-bishydroxymethylfuran in the medium did not affect cell growth or ethanol production. Biotransformation of HMF to less inhibitory compounds by P. stipitis KCTC 7228 could enhance overall fermentation of seaweed hydrolysates to ethanol.