원문정보
초록
영어
Seaweed is a third-generation biomass that can be used in bioenergy production and has many advantages. The seaweed grows quickly, is lignin-free and is not used as a food crop. The red seaweed, Euchema sponosum, is cultivated in Indonesia. The first step, seaweed slurry was pretreated by thermal acid hydrolysis and enzyme treatment. Optimal pretreatment condition was determined with 11% (w/v) solid content of seaweed and 150 mM sulfuric acid at 121℃ for 40 min. And enzyme saccharification was carried out Celluclast 1.5L and Viscozyme L at pH 4.5, 40℃. However, E. spinosum produced Five-HMF of 6 g/L after thermal acid hydrolysis. To removed 5-HMF, in addition of active charcoal was carried out in the range of 3-6% at room temperature for 2 hour. 5-HMF was reduced from 6 g/L to 1 g/L on 6% active charcoal. The ethanol fermentation was carried out using SHF with Kluyveromyces marxianus KCTC 7150. The ethanol fermentation from 5-HMF removed E. sponosum hydrolysate produced 18 g/L using K. marxianus for 48 h. On the other hand, ethanol fermentation was prolonged when 5-HMF existed.