원문정보
초록
영어
Oleaginous yeast has attracted great attention as high potentials for the biodiesel production because it grows fast and its final cell density is high. However, the oleaginous yeast utilizes organic substrates for its growth, and it causes the price of biodiesel to rise sharply [1]. This research focuses on optimizing conditions for the cultivation of oleaginous yeast Cryptococcus sp, which was isolated from fermented seafood, using low-cost substrate, Jerusalem artichoke.The major component of Jerusalem artichoke is inulin, which is composed of fructose units terminated by a glucose unit [2]. It has excellent environmental adaptability, is easy to cultivate, and cannot be digested by the human enzyme system. For these reasons, Jerusalem artichoke seems to be a potential feedstock candidate [3]. To utilize Jerusalem artichoke as a microbial substrate, pretreatment process is absolutely needed. The pretreatment factors, such as temperature, reaction time, and the concentration of nitric acid were optimized through response surface methodology (RSM). An optimal pretreatment condition was at 101℃, 0.7% of nitric acid, and for 10 min, which resulted in 53.4% of fructose yield.