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영어

In this talk, I will present the use of physically modified microfluidic channels towards various biomedical applications including single cell analysis and organ chips. The physical modification can be realized either by embedding physical micro/nanostructures or including a topographically patterned substrate at micro- or nanoscale inside a channel. To enable micro/nanofabrication with microfluidics, a simple capillarity-driven lithographic method termed “capillary force lithography” is introduced for fabricating micro/nanostructures onto a surface or inside a microfluidic channel. In the first part, hollow micro-wells are integrated inside a microfluidic channel to capture cells at single cell level. Using this microfluidic platform, we carried out programmable time-course live-cell imaging aided by fluorescent image processing. In the second part, the method is applied for mimicking various aspects of in-vivo environments for cells or tissues. Two organ chips are presented for potential drug screening and advanced tissue engineering applications: heart and kidney chips.