earticle

영어

Structural modified siRNAs with a higher number of siRNAs can easily form a stable nanoparticle structure with thiolated biocompatible polymers and thiolated proteins through charge-charge interaction and chemical crosslinking, simultaneously. To investigate the potential use of new siRNA delivery system, we suggested the new studies to increase the size of siRNA via structural modification of the siRNA itself. The attempts include several methodologies such as hybridization, chemical polymerization, and micro- and nano-structurization of siRNA. Due to its increased charge density and flexibility, the structured siRNA can produce highly condensed and homogenous polyplexes compared to the classical monomeric siRNA. As a result, stable and compact siRNA polyplexes can enhance serum stability and target delivery efficiency in vivo with desirable biodistribution