earticle

영어

First, we present the development of microfluidic scaffolds that are formed by embedding microfluidic networks directly within biomaterials (alginate and collagen). Microchannels directly embedded within hydrogels enable spatiotemporal control and remodeling of cellular microenvironment. Emerging interdisciplinary science/engineering fields allow for the development of micro-/nano-scale sensing and detection technologies that can serve as enabling tools for biological applications. We present the development of polymeric nanoparticles loaded with an oxygen-sensitive phosphor to perform quantitative measurements of the concentration of oxygen within 3D tissue cultures in vitro and blood vessels in vivo. We synthesized poly(urethane acrylate nonionomer) (PUAN) nanoparticles incorporated with a ruthenium phosphor. Finally, we present the quantification of unmodified full length-transcripts using poly(ethylene) glycol diacrylate (PEGDA) hydrogel microparticles. We show that PEG600 served as an effective porogen to allow for the capture of large mRNAs. We also demonstrate a 3-plex mRNA detection, without cross-reactivity, using shape-encoded “intraplex” hydrogel microparticles.