원문정보
초록
영어
Recent advances in directed evolution techniques have enabled us to engineer biocatalysts for broadening their industrial uses. Nevertheless, most engineering strategies still require labor-intensive and complicated procedures to obtain tailor-made enzymes to meet industrial demands. Instead of conventional rational design and directed-evolution approaches, here we used a semi-rational approach based on amino acid sequence comparisons of homologous enzymes for engineering their intrinsic properties (i.e., pH optimum, thermostability, metal requirement etc.). Semi-rational ‘consensus concept’ led us to engineer the physicochemical properties of L-arabinose isomerases (AIs) for the production of a novel and natural sweetener, D-tagatose at elevated temperatures. Our case studies with a variety of AIs suggest that such a simple semi-rational strategy could be very effective and powerful for engineering AIs.