earticle

영어

Lysosomes are heterogeneous cell organelles consisting of vacuoles that vary in size, form, and density, and lysosomes release lysosomal enzymes to digest intracellular and extracellular waste material. Lysosomal enzymes degrade bacteria cell walls, demonstrating the antimicrobial activity of lysosomes and they have anticancerous effect when mammalian cells progress apoptosis. Lysosomes are not silent organelles, but dynamic organelles in which lysosomal enzymes are easily integrated or released when exposed to stressful conditions, especially oxidative stress. Understanding in vitro lysosomal function in cells following hydrogen peroxide (H2O2), 6-hydroxydopamine (6-OHDA), or UVB irradiation may elucidate lysosomal mechanisms associated with oxidative stress and lysosomal activation. We exposed HeLa cell lines to three oxidative causing agents and isolated lysosomes from HeLa cells exposed to these stressors. It was found that the lysosomes have antimicrobial activity against seven different microorganisms, including E. coli, and oxidative stress-induced lysosomes showed enhanced antimicrobial activity compared to those from normal lysosomes. These results suggest the possibility that lysosomal alterations during starvation may induce conditions that activate lysosomes for future development of efficient antimicrobial agents. This study suggests a new methodology for enhancing lysosomal activity in a lysosome-source strain through special treatment, such as starvation.