원문정보
Evaluation of Antioxidant and Anti-Inflammatory Activities of Ascidian Tunic Carotenoids As a Source of Color Cosmetics
초록
영어
Carotenoids are fat-soluble red-orange colored pigments found in plants and seafood-derived products, including algae, seaweeds, and fish muscle. In this study, we have demonstrated the molecular mechanism underlying the antioxidants and anti-inflammatory properties of ascidian tunic carotenoids using mouse macrophage cell line (RAW 264.7).
Cell viability was not affected by treatment of carotenoids < 10 μg/mL. This treatment also showed negative inhibition on lipopolysaccharide (LPS)-stimulated nitric oxide (NO) and cyclooxygenase-2 (COX-2). The DPPH radical scavenging activity of carotenoids was 47.2% at 100 mg/mL. It also has a potential reducing power (1.025) comparable with ascorbic acid (1.584). The ascidian tunic carotenoids would make a candidate for the commercially interesting biologically active cosmetic pigments.
목차
1. 서론
2. 재료 및 방법
2.1. 카로테노이드 색소추출 및 함량 측정
2.2. 페놀화합물 측정
2.3. DPPH 라디칼 소거활성 측정
2.4. 환원력 측정
2.5. 세포배양 및 세포독성 측정
2.6. Nitric oxide (NO) 생성 억제능 측정
2.7. COX-2 저해 활성 측정
2.8. 통계학적 분석
3. 결과 및 고찰
3.1. DPPH 라디칼 소거 활성능
3.2. 환원력에 의한 항산화 효과
3.3. RAW 264.7 cell line에 대한 독성시험
3.4. NO 생성 억제 활성
3.5. COX-2의 저해 활성
4. 결론
감사
REFERENCES