원문정보
Study on Online Monitoring of Dissolved Oxygen, pH and CellConcentration in E. coli Cultivation Processes Using MABOOMSTM
초록
영어
Dissolved oxygen, pH and cell concentration have been online monitored in cultivation processes with Escherichia coli by using a MABOOMSTM (microplate-based bioreactor with optical online monitoring systems). Fluorescent sensing membranes containing Ru (dpp)3 2+ or HPTS were prepared with GA sol-gel matrix and coated into a well of a 24-well microplate. Fluorescence intensity was measured and correlated to the dissolved oxygen or pH. Cell concentrations were also online monitored by measuring optical reflectance at 650 nm. A well of a 24-well microplate could also be divided into 4 parts, each of which was coated with fluorescent sensing membranes for the detection of dissolved oxygen or pH.
The 24-well microplate coated with fluorescent sensing membranes or a 4-divided sensing membrane. was used to online monitor the dissolved oxygen, pH and cell concentration during E. coli cultivations. The online monitoring results showed the characteristics of cell growth in cultivation processes very well.
목차
1. 서론
2. 재료 및 방법
2.1. 마이크로플레이트 기반 생물반응기 시스템(MABOOMSTM)
2.2. 광학 센서막
2.3. 미생물 및 배지
2.4. 분석
3. 결과 및 고찰
3.1. 용존산소의 온라인 모니터링
3.2. pH의 온라인 모니터링
3.3. 세포농도의 온라인 모니터링
3.4. 용존산소, pH 및 세포농도의 동시 온라인 모니터링
4. 결론
감사
REFERENCES