원문정보
Anti-Inflammatory Effect of Chloroform Extract from Potentilla chinensis
초록
영어
In this study, we investigated the anti-inflammation effect of Potentilla chinensis (PC) on Raw264.7 macrophage cells. Ethanol extract of PC decreased the production of nitric oxide (NO) in LPS-stimulated RAW264.7 cells. Ethanol extract was fractioned by n-hexane, chloroform, ethyl acetate, n-butanol, water and each fraction was tested for inhibitory effects on inflammation. Among the sequential solvent fractions, PC chloroform extracts (50, 100, 300, and 500 μg/mL) significantly suppressed LPS-stimulated production of NO. During the entire experimental period, 200 and 300 μg/mL of PC chloroform extracts had no cytotoxicity. LPSinduced NO and prostaglandin E2 (PGE2) production were inhibited by PC chloroform extracts up to 50% and 90% of these productions, respectively. PC chloroform extracts reduced the expression of iNOS and COX-2 gene. These results suggest that PC chloroform extracts exhibit strong effects of anti-inflammation and can be a potential candidate in the treatment of acute and chronic inflammatory diseases.
목차
1. 서론
2. 재료 및 방법
2.1. 시료 및 시약
2.2. 시료 추출
2.3. 세포 배양
2.4. MTT assay에 의한 세포 생존율 측정
2.5. NO와 PGE2 생성량 측정
2.6. 염증 유발 효소 발현 측정
2.7. 통계처리
3. 결과 및 고찰
3.1. 수율
3.2. NO와 PGE2 생성 억제효과
3.3. 염증유발 효소 발현 측정
4. 결론
감사
REFERENCES