원문정보
초록
영어
We investigated the effects of swimming and treadmill exercise on the level of nerve growth factor (NGF) protein and neurogenesis in the hippocampus, and cognitive function of adult rats over a period of 8 weeks. We divided 144 male Sprague-Dawley rats into 3 groups: (1) a control group (COG; total n = 48, n = 8 for each time-point), (2) a swimming exercise group (SEG; total n = 48; n = 8 for each time-point), and (3) a treadmill exercise group (TEG; total n = 48, n = 8 for each time-point). The SEG and TEG were made to perform their respective exercise type for 5 days per week over a period of 8 weeks. The level of NGF on the second day, and after the first, second, and fourth week increased significantly in the SEG and TEG, compared to the COG (2 days, 1 week, 2 weeks: p < 0.001; 4 weeks: p < 0.01 ). Specifically, a significant increase was observed in the SEG at the 2-day, 2-week, and 4-week time-points. A significant difference in the number of BrdU-positive cells was found between groups at all time-points (6 months: p < 0.05; 2 days, 2 weeks, 4 weeks, and 3 months: p < 0.01; 1 week: p < 0.001). Specifically, a significant increase was observed in the SEG at the 1-week and 4-week time-points. The number of NeuN-positive cells in the SEG increased significantly at all time-points (2 days, 1 week, 2 weeks, 4 weeks, 3 months, and 6 months: p < 0.001). The number of DCX-positive cells between groups was also significantly different at all time-points, except for the fourth week, (6 months: p < 0.05; 2 days, 1 week, 2 weeks, 3 months: p < 0.001). Specifically, a significant increase was observed in the SEG at the 2 weeks and 3-month time-point. These results show that regular exercise in adult rats increased the level of NGF in the hippocampus, increased the number of newly proliferated nerve cells, and extended the period of neuron survival and maintenance. Furthermore, this phenomenon was more apparent when the exercise form was swimming.
목차
Ⅱ. 연구방법
1. 실험대상
2. 실험절차
3. 조직채취
4. 분석항목 및 방법
5. 자료처리
Ⅲ. 결과
1. 운동형태에 따른 NGF 수준의 변화
2. 운동형태에 따른 BrdU-labeled cells의 변화
3. 운동형태에 따른 인지기능의 변화
Ⅳ. 논의
Ⅴ. 결론
참고문헌
ABSTRACT