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Purpose: We investigated the relationship between patient safety culture and safety care activity, and identified factors for safety care activity of entry-level nurses. Methods: The subjects of the study were 204 entry-level nurses working at five general and advanced hospitals with over 500 beds located in C and D City. The data were analyzed using descriptive analysis, t-test, ANOVA, Pearson’s correlation and hierarchical multiple regression with SPSS/WIN 18.0 program. Results: Perception of patient safety culture had significant correlations with safety care activity. Multiple regression analysis showed that demographic variables of quality of nursing on patient safety and health state of entry-level nurses affected safety care activity. In addition, patient safety culture in ward and communication affected safety care activity. These factors explained 38% of variance. Conclusion: Based on the results of the study, patient safety culture promotion strategies to facilitate supportive work environment and effective communication are needed.