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연구논문

정상모의 퍼머넌트 웨이브 시술 시 분자량 분포도가 다른 갯지렁이(Marphysa sanguinea ) 단백질 분해물의 전처리 효과

원문정보

Pretreatment Effect of Different Molecular Weight Lugworm Protein Hydrolyzate in Treating Permanent Wave of Virgin Hair

신지은, 강상모

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

This study was intended to clarify pre-treatment effect of the lugworm autolysates of which molecular weight distribution are different respectively in treating permanent wave on normal hair. We added the protease crude enzyme extract gotten by cultivating Bacillus subtilis K-54 separated from cheonggukjang when making the lugworm autolysates. In the meantime, we made the molecular weight distribution of lugworm autolysates became different through making the additive amount of protease crude enzyme became different. We observed the molecular weight distribution of lugworm autolysates using SDS- PAGE, and measured hair thickness, tensile strength, density analysis by methylene blue dyeing, wave formation & maintenance strength with the protease crude enzyme (0.112 unit) after carrying out the pre- treatment on each autolysates. In the event of making the lugworm autolysates, the highest rate for the permanent effect was shown at MbB50 (0.8950×50 unit) of which protease per 1g lugworm was added by about 50 times. but effect was shown at MbB30 (0.0895×30 unit) in wave long-lasting. As the result of study, for securing quality as a pretreatment agent in treating permanent wave on normal hair, it was thought that it is more important to make the earthworm protein became the decompression substances with the proper size than to make them became the low molecular protein.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구재료 및 방법
  1. 갯지렁이
  2. 시험균주
  3. 시료모발
  4. 갯지렁이 분해물 제조
  5. Protease 효소 활성측정법
  6. 갯지렁이 분해물의 전처리 후 퍼머넌트 웨이브 시술
  7. 모발의 굵기 측정
  8. 모발의 인장강도 측정
  9. Methylene blue 염색법을 이용한 모발 밀도 측정법
  10. 웨이브 형성력
  11. 웨이브 지속력
 Ⅲ. 연구결과 및 고찰
  1. SDS-PAGE를 이용한 갯지렁이 단백질 분자량 분포도
  2. Protease 처리한 정상모의 모발 굵기 변화
  3. Protease 처리한 정상모의 인장강도 변화
  4. Methylene blue 염색법을 이용한 모발 밀도 측정
  5. Protease 처리한 웨이브 형성력
  6. Protease 처리한 웨이브 지속력
 Ⅳ. 결론
 참고문헌

저자정보

  • 신지은 Ji-Eun Shin. 건국대학교 대학원 생물공학과
  • 강상모 Sang-Mo Kang. 건국대학교 미생물공학과

참고문헌

자료제공 : 네이버학술정보

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