원문정보
Inhibition of Adipogenesis in 3T3-L1 Adipocytes with Ethanol Extracts of Saururus chinensis
초록
영어
1)Obesity increases the risk of many adult diseases, such as atherosclerosis, diabetes, hypertension, ischemic heart disease and breast cancer. Inhibition of adipogenesis is an effective way in the anti-obesity management. Because of main components of Saururus chinensis is flavonoid, it has been showed some improvement by its antioxidant effects on the atherosclerosis, heart disease and diabetic hyperlipidemia. But mechanism of anti-obesity effect of S. chinensis is not clear. We investigated the effects of ethanol extracts of S. chinensis on adipogenesis in the 3T3-L1 pre-adipocyte. The 3T3-L1 cell line is commonly used to study adipogenesis in vitro. In this study, ethanol extracts of S. chinensis significantly decrease the lipid accumulation in the 3T3-L1 cells proved by measuring triglyceride contents and Oil red O staining. The proposed mechanism of inhibition of adipogenesis in the 3T3-L1 cells with ethanol extracts of S. chinensis is down-regulation of transcriptional factors and adipocytespecific genes such CCAAT/enhancer binding protein α (C/EBPα) and Peroxisome proliferator activated receptor γ (PPARγ) in concentration dependent pattern. These results suggest that ethanol extracts of S. chinensis inhibits adipognesis in the 3T3-L1 cells and can be used as a safe and efficient natural substance to manage anti-obesity.
목차
1. 서론
2. 재료 및 방법
2.1. 시료의 추출
2.2. 지방세포 배양 및 분화
2.3. MTS 분석
2.4. Triglyceride (TG) 분석
2.5. Oil Red O 염색
2.6. Western blot
2.7. Reverse transcriptase polymerase chain reaction (RT-PCR)
2.8. 통계분석
3. 결과 및 고찰
3.1. 3T3-L1의 분화에 대한 삼백초의 효과
3.2. 지방세포분화 전사인자와 표지인자의 발현에 대한 삼백초의 효과
감사
References