원문정보
The Injured Starfish Extract Inhalation Effects of Anti-inflammation and Anti-oxidants Enzyme during Indirect Cigarette Smoking
초록
영어
Cigarette smoking (SM) is considered to be well known environmental toxin which contributes to the onset of various diseases. SM cause direct lungs damage, activate lungs inflammatory responses, and in some cases leads to the development of lung cancer. Cytokines in injured starfish (Asterina pectinifera) is the potential changes in its expression during the regeneration process. Especially, expression of TGF-β1 has increased in arm cut starfish extract after eight days. Also, starfish including saponin like the ginseng. Saponin is widely used in the world because of some effective pharmacological activities. Therefore, the current study was designed to elucidate the pharmacological activities of starfish extract against cigarette smoking induced damage in cell line and pulmonary tissue. We investigate that the effect of eight days starfish extract after arm cut (8d) and intact starfish extract on cell line and mouse lung injury by SM. In cell proliferation analysis, although cigarette smoking extract (CSE) was co-treated, the higher proliferation ability is shown in 8d treatment than intact starfish extract. 8d and intact starfish extract was directly transported to pulmonary cells through respiratory organ by nebulizer inhalation. In this case of cigarette smoking, the pulmonary structure was damaged and functions become abnormal. However, 8d treated groups showed similar with the control group compared with SM group. Among them, 8d was proved to be more effective than intact starfish extract. These results demonstrate that 8d could more protect pulmonary structure and function than intact starfish extract against cigarette smoking by ginseng like saponin and regulation of inflammatory cytokines.
목차
1. 서론
2. 재료 및 방법
2.1. 시약 및 장비
2.2. 재료 및 처리조건
2.3. 불가사리 추출
2.4. 담배연기 추출
2.5. 세포주 및 실험조건
2.6. 실험군 및 실험조건
2.7. In Vitro 실험
2.8. In Vivo 실험
2.9. 폐의 채취 및 분리
2.10. Hematoxylin & Eosin stain (H&E stain)
2.11. 통계 처리
3. 결과 및 고찰
3.1. 세포 증식효과
3.2. 세포내 단백질 활성 측정
3.3. 체중의 변화
3.4. Catalase 활성
3.5. 지질과산화 측정
3.6. 혈액성분의 변화
3.7. 단백질 활성 측정
3.8. 폐 조직의 변화
4. 결론
References