표고균사 갈변과 관련된 BCR (Brown Color Repressor) 유전자 분리


BCR (Brown Color Repressor) gene isolation related to mycelial browning of Lentinus edodes

김영호, 박수철, 전창성, 유창현, 성재모, 공원식

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Recently sawdust cultivation of Shiitake mushroom (Lentinula edodes) is increasing. It is important to make mycelia to be brown on the substrate surface. This browned surface in sawdust cultivation plays an important role like as artificial bark of the oak log, which protects the other pests and suppresses water evaporation in the substrate. In order to isolate genes which related to brown color formation, differential display method was used. Two cDNA fragments obtained by DD-PCR were 1.2 and 1.6kb and these were expressed in white colored mycelia from L. edodes, but not brown colored mycelia. Partial sequencing of these cDNA fragments showed that the 1.6kb cDNA had 100% identity with the microsatellites gene from Dugenia polichroa. However, the other 1.2kb cDNA fragment had poly T tail on 3’ region of partial open reading frame on 5’ region. The new primer designed based on the sequence of 1.2kb cDNA was constructed. RT-PCR analysis using the newly designed 0.12kb cDNA specific primer showed that the gene was only expressed in white color mycelia, but not in brown color mycelia. Sequence analysis of 5’ region of this 1.2kb cDNA revealed that this gene contained partial open reading frame consisted of 110 amino acid. Homology search using DNASIS database showed that this gene had high sequence homology of 66.7% in DNA level and 69.2 % in amino acid level with dTDP-glucose 4,6-dehydratases gene from Arabidopsis thaliata. The dTDP-glucose 4,6- dehydratases gene was known to be function to have tolerance with oxidation stress. These results strongly suggest that this gene isolated from white mycelia of L. edodes might have a function of repressor against mycelia browning. Therefore I designated this gene as BCR (Brown Color Repressor) gene.


 재료 및 방법
  갈변관련 유전자 탐색체계
  균사로부터 total RNA의 분리
  Differential display PCR
  갈변관련 유전자의 검정
  DNA염기서열 결정
  게놈 DNA의 분리
  RAPD 분석
 결과 및 고찰
  염기서열 분석
  1.2kb cDNA의 색소형성관련성 확인
  1.2kb 유전자의 확인
  1.2kb 유전자의 발육시기별 발현 양상
  BCR 유전자의 염기서열 분석


  • 김영호 Young-Ho Kim. 국립원예특작과학원 버섯과
  • 박수철 Soo-Cheol Park. 국립농업과학원
  • 전창성 Chang-Sung Jhune. 국립원예특작과학원 버섯과
  • 유창현 Chang-Hyun You. 국립원예특작과학원 버섯과
  • 성재모 Jae-Mo Sung. 머쉬텍
  • 공원식 Won-Sik Kong. 국립원예특작과학원 버섯과


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