초록
영어
Pleurotus ostreatus, the oyster mushroom, is one of the most important edible mushrooms. It is especially susceptible to bacterial blotch disease, which is caused by Pseudomonas tolaasii. In order to develop bacterial blotch disease-resistant transgenic mushroom, NUECIN cDNA, a gene for an antibacterial peptide cloned from Bombyx mori, was overexpressed in Pleurotus ostreatus. NUECIN cDNA was fused to the β-TUBULIN promoter of oyster mushroom and co-transformed with the pTRura3-2 vector into the uracil auxotrophic mutant strain. Twelve transformants containing the Nuecin gene were identified by genomic PCR and Southern blot analysis. NUECIN gene expression was confirmed by Northern blot analysis. Three transformants showed the transcriptional expression of the gene. However, we could not detect expression of the protein in the transformants. This study showed the possibility of transgenic mushroom development for disease resistance.
목차
Introduction
Materials and methods
Strains and plasmids
Vector constructs
Transformation
Genomic DNA isolation and Southern blot analysis
Northern blot analysis of transformants
Western blot analysis of transformants
Results
Isolation of transformants co-transformed withpTubnuecin and pTRura3-2
NUECIN cDNA was transcriptionally expressed intransformants
Transformants did not show antibacterial activityagainst P. tolaasii
NUECIN protein was not detected in the transformants
Discussion
적요
References