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rDNA-ITS 및 CAPS 분석에 의한 꽃송이버섯 (Sparassis crispa) 수집균주의 계통분류학적 특성구분

원문정보

Phylogenetic relationships of medicinal mushroom Sparassis crispa strains using the rDNA-ITS and CAPS analysis

정종천, 이명철, 전창성, 이찬중, 신평균

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

This study was carried out to analyze the genetic relationships among 22 strains of Sparassis crispa, which were collected from various regions of worldwide. The cleaved amplified polymorphic sequence were obtained from the ribosomal DNA ITS regions of each strain. Based on the sequence analysis, the presence of five different groups were observed. Most strains shared the high nucleotide sequence similarity (about 90%) to each other, except only one strain, KACC50866. Nucleotide sequence similarity of KACC50866 was below 10% to other strains, indicating the genetic relatedness of strain KACC50866 was low compared to other strains. More works such as mitochondria genome analysis should help to determine the precise genetic diversity of S. crispa strains.

목차

ABSTRACT
 서론
 재료 및 방법
  공시균주
  분자생물학적 유연관계 분석
  제한효소 절단 분석
  PCR 산물의 클로닝 및 염기서열 분석
 결과 및 고찰
  꽃송이버섯 수집균주의 ITS 영역 구조 분석
  제한효소 절단 분석
  ITS 영역의 염기서열을 이용한 꽃송이버섯속균의 유연관계
 적요
 인용문헌

저자정보

  • 정종천 Jong-Chun Cheong. 농촌진흥청 국립원예특작과학원 버섯과
  • 이명철 Myung-Chul Lee. 국립농업과학원 농업유전자원센터
  • 전창성 Chang-Sung Jhune. 농촌진흥청 국립원예특작과학원 버섯과
  • 이찬중 Chan-Jung Lee. 농촌진흥청 국립원예특작과학원 버섯과
  • 신평균 Pyeong-Gyun Shin. 농촌진흥청 국립원예특작과학원 버섯과

참고문헌

자료제공 : 네이버학술정보

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