환경조건이 표고톱밥배지의 갈변에 미치는 영향


The effect of environmental condition to the mycelial browning of Lentinula edodes (Berkeley) Sing. during sawdust bag cultivation

김영호, 전창성, 박수철, 유창현, 성재모, 공원식

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Recently sawdust cultivation of Shiitake mushroom (Lentinula edodes ) is getting increased because log cultivation is getting difficult to get oak logs. It is important to make mycelia browning on the substrate surface in sawdust cultivation. This browned surface plays an important role like as artificial bark of the oak log, which protects the other pests and suppresses water evaporation in the substrate. The period for mycelia browning is so long that the sawdust cultivation of Shiitake mushroom can not spread well into the mushroom farms. In this article we would like to discuss about the effect of environmental condition to the mycelial browning during sawdust bag cultivation for the To reduce the period required for browning of substrates, sawdust substrates was illuminated light with difference intensity. One hundred Lux light illumination was needed for producing normal yield of fruit body but fruit body yield was low and abnormally shaped fruit body was produced when cultured under the dark condition of incubation. Illumination over 200lux is necessary for the successful browning of substrates during incubation. Optimum incubation temperature for browning of substrates and fruiting was 25℃. The treatment of cotton plug with different size to identify the effect of aeration on the browning of substrates and fruiting showed rapid mycelial growth and reduced the periods for browning as the size of cotton plug was bigger. However, yield of fruit body was the highest at 16mm diameter cotton plug as compared to 20mm of that. CO2 content in vessel of substrates was low as the size of cotton plug was bigger during incubation. CO2 content during incubation of substrate was highest in periods between 8 week and 14 week after inoculation of shiitake when substrate was changed color into brown. C2H4 content in vessel with substrates was highest at 8mm diameter cotton plug and it was increased by order of 12, 16, 20, 0, 4 mm diameter cotton plug during substrate incubation. Sawdust substrate was soaked in cold water for different time to identify soaking effect of sawdust substrate on fruit body yield and activities of enzymes in these substrates were investigated. The fruit body yield was increased up to 40% by soaking substrates in comparison with unsoaked substrates. The soaked substrates showed 165, 175g/1,000ml at treatment of 4 and 15 hours, respectively. Cellulose activities in soaked substrates were not changed with soaking time, but activities of laccase, lignin degradation enzyme, were drastically increased up to 4 times in comparison with unsoaked substrates.


 재료 및 방법
  광조사에 따른 배지의 변화
  배지내 통기량의 조절
  배지내 CO2 와 C2H4의 변화량 조사
  톱밥배지의 침수
  세포외 효소의 측정
 결과 및 고찰
  표고 톱밥배지의 배양시 광조건
  표고 톱밥배지의 배양온도 조건
  표고 톱밥배지의 배양중 통기조건
  표고 톱밥배지의 침수에 따른 자실체 특성변화


  • 김영호 Young-Ho Kim. 국립원예특작과학원 버섯과
  • 전창성 Chang-Sung Jhune. 국립원예특작과학원 버섯과
  • 박수철 Soo-Chul Park. 농촌진흥청 연구정책과
  • 유창현 Chang-Hyun You. 산림버섯연구소
  • 성재모 Jae-Mo Sung. 강원대학교
  • 공원식 Won-Sik Kong. 국립원예특작과학원 버섯과


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