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영어

Agaricus bisporus is a nutritious edible fungus that is cultivated worldwide. Because of its secondarily homothallic life cycle, 90% or more of the basidia lining the gills at fruit bodies are predominantly two-spored, with each spore receiving two of the four meiotic nuclei. Such homothallic system results in scarcity of monokaryotic basidiospores in its progeny but the availability of uninucleate self-sterile homokaryons is a pre-requisite for producing hybrids in breeding program. This experiment screened 64 single spore isolates collected from CN0210-021, which is white button mushroom cultivated in Chungnam area. They were germinated on PAD meduium at 25℃. After 30 days of incubation colony diameter and morphology type of each isolates was determined. Based on growth rate, 5 strains showing very slow growing morphology than that of parental heterokaryotic strain were selected. Out of 5 slow growing isolates, 2 of them fruited and the rest 3 isolates did not fruit, which were considered as putative homokaryons. These data will provide the outcrossing sources that could be used in commercial breeding programs later. However, in the identification of homokaryotic strains, there is no direct morphological distinguishing features such as a clamp connection that would permit the reliable sorting between homokaryons and heterokaryons. Traditional method to verify a homokaryon thorough the fruiting trial is also time-consuming and not perfect. Therefore, to accelerate the breeding of Agaricus bisporus, quick and reliable methods such as
molecular markers to identify the infrequent homokaryons from heterokaryons are needed to be carried out.