원문정보
초록
영어
The sterile form of Inonotus obliquus is used for preparation of the medicine befungin that is effective in the treatment of gastritis, gastric ulcers, and several tumors. The fungus is known to be produced mainly on the stems of Betula platyphylla var. japonica that grows at high altitudinal (above 1,100 m) region in Korea. But, we found the mushroom on the stem of Betula costata at Mt. Jumbong in Korea. We isolated a pure culture of the fungus from the stem of B. costata by use of potato dextrose agar (PDA) medium with streptomycin. We could isolate the fungus from plant's tissue filled with hyphae, but not from other parts. The spore collected from the sclerotia showed 6.0∼10.0 × 4.5∼ 6.0㎛ in diameter, and the hypha was 2.5∼5.0㎛ in thickness. The colony showed irregular features and scattered yellow color at the center as the culture ages. We could find brownish setae at the yellow region of colony at 20 days of culture, and the size ranged 4∼6 × 100∼420㎛. The oatmeal agar (OA) provided best growth for I. obliquus among five media (CDA, CMA, MA, OA and PDA). Optimum temperature ranged 25∼30℃, and optimum pH was relatively alkaline with the range of pH 8.0∼9.5.
목차
재료 및 방법
차가 균핵 채취
균 분리
동정
배양특성 조사
결과 및 고찰
균 분리 및 동정
배양특성
적요
참고문헌