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약용버섯의 계통분류 및 국내유통 Inonotus속내 종간 구별을 위한 신속동정법 개발

원문정보

Phylogenetic analysis of the medicinal mushroom and taxonomical positions of their commercial products

김성윤, 정민정, 김기영, 박재민, 김문옥, 문동오, 이태호, 이재동

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초록

영어

The Aphyllophorales is a large order containing about 2,000 known species. Many of these are the bracket and coral fungi. The vast majority of these fungi are saprophytic on the plant debris. Many species are significant in decomposing plant remains, as they are able to digest cellulose or lignin that occurs in plant cell walls. Many of these fungi have been involved in everyday human affairs. A few were used medicinally by the Greeks and Romans as a remedy for many complaints, including colic, fractured limbs and bruises. Other bracket fungi have been used as curry combs for horses, as snuff, as razor strops and as a source of dye for clothing. The texture of the basidiocarp may be similar to that of cork, wood, leather, paper, or cartilage. Unlike the basidiocarps of the Order Agaricales, the basidiocarps of the Aphyllophorales are not fleshly and moist. Division of the members of the Aphyllophorales into genera was originally made on the basis of gross morphology of the basidiocarp and hymenium and Donk(1964) recognizes 22 families in this order. The species and genus whose typical in Aphylloporales were listed in Table. with related information. The ITS region sequence of some genus were found by BLAST search. Sequences retrieved from GenBank were visually aligned by the program CLUSTAL G. As a result, the medicinal mushroom was separated in four groups. In this multiple alignment, the sequence analysis among Fomes group, Inonotus group and Phellinus group showed high genetic similarity except Hericium group and Sparassis group.

목차

ABSTRACT
 재료 및 방법
  균주 분리 및 배양
  배양조건
  DNA 추출
  PCR증폭
  PCR 산물 정제
  염기서열 분석
  유연관계 분석
 결과 및 고찰
  rDNA 염기서열 비교분석
  국내 약용버섯의 계통분류학적 유연관계 분석
  시판 Inonotus의 분류학적 위치
  종 특이적인 유전자 탐침의 개발 및 검증
 적요
 감사의 글
 참고문헌

저자정보

  • 김성윤 Cheng-Yun Jin. 부산대학교 자연과학대학 미생물학과
  • 정민정 Min-Jung Jeong. 부산대학교 자연과학대학 미생물학과, 경상북도 산림환경연구소
  • 김기영 Gi-Young Kim. 동의대학교 한의학임상연구센터
  • 박재민 Jae-Min Park. 부산대학교 자연과학대학 미생물학과
  • 김문옥 Mun-Ok Kim. 부산대학교 자연과학대학 미생물학과
  • 문동오 Dong-Oh Moon. 부산대학교 의과대학 미생물학 및 면역학교실
  • 이태호 Tae-Ho Lee. 부산대학교 자연과학대학 미생물학과
  • 이재동 Jae-Dong Lee. 부산대학교 자연과학대학 미생물학과

참고문헌

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