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논문검색

Screening of Fruiting Body Formation-Specific Genes from the Medicinal Mushroom Cordyceps militaris MET7903

원문정보

약용버섯 번데기 동충하초 MET7903의 특이적 자실체형성 유전자의 선별

Bangung Yun, Ki-Chul Chung

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

This study was carried out to screen the fruiting body formation-specific genes from the medicinal mushroom Cordyceps militaris. A cDNA synthesized using total RNA from 4 stages of mushroom development, mycelium, primordium, immature fruiting body and mature fruiting body. Differential expression gene screening was performed by DD- PCR(Differential Display Arbitrary Primer PCR) with cDNA, we sequenced partial 6 genes using pGEM cloning vector. The DNA Sequence of the six DD-PCR products derived from differentially expressed genes was compared to that in the GenBank database by using the NCBI BLAST search to identify similarities to known sequences. Sequence analysis showed that six of DD-PCR products have unknown sequence.

목차

ABSTRACT
 MATERIALS AND METHODS
  Strain and Culture condition
  Preparation of totalRNA
  Synthesis of cDNA
  Differential Display an Arbitrary Primer PCR
  Cloning of partial fragment
 RESULTS AND DISCUSSION
 적요
 REFERENCES

저자정보

  • Bangung Yun 윤방웅. Dept. Biotechnology, School of Biological Sciences and Technology
  • Ki-Chul Chung 정기철. Biotechnology Research Institute, Chonnam National University, Gwangju 500-757, Korea

참고문헌

자료제공 : 네이버학술정보

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