원문정보
초록
영어
To develop neohaplonts for Ganoderma breeding, protoplasts were isolated from dikaryotic mycelium and regenerated. Selection rate of neohaplonts varied between ASI7074, ASI7091, ASI7094, ASI7100 and ASI7115, showing 5.24% on the average. Auxotropic mutants from Ganoderma monokarions were recovered by UV irradiation on protoplasts. Protoplast survival rates were 1.9% ASI 7074, 0.17% ASI 7091, and zero percent ASI 7100 using 300 second irradiation. Four auxotrophic strains were recovered from 1,536 colonies screened that will be further utilized for protoplast fusion and transformation.
목차
재료 및 방법
공시균주 및 배지
Neohaplonts 선발
돌연변이 유발
영양요구주의 선발 및 유전표지 확인
결과 및 고찰
Neohaplonts 선발
돌연변이 유기
영양요구주 확인
적요
감사의 글
참고문헌