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논문검색

P-115

Sialic acid lectin (MC-sialec) produced by Perkinsus olseni-infected Manila clam (Ruditapes philppinarum)

원문정보

Youngmi Kim, Mausumi Adhya, Kwang-Sik. Choi, Younghun Yu, Moonjae Cho

한국당과학회 한국당과학회 학술대회 ACGG 2012 Conference 2012.10 pp.110-110
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

Lectin has been played a key innate immune parameter in invertebrate host defense. Previously, we reported a novel sialic acid specific lectin (MC-sialec) cloned from EST sequence of Manila clam hemocytes infected with Perkinsus olseni. The lectin was expressed in E. coli M 15 and purified by Ni-NTA His binding resin matrix for antibody production. Lectin specific antibody was raised in mice. The presence of this lectin in different tissues of Perkinsus infected manila clam was studied by both Real Time-PCR and immunohistochemical localization assay. The results showed that upon infection, expression of MC-sialec was induced and expressed in foot, gill, adductor, mantle, hemocyte, stomach, intestine and digestive gland, siphon, palp and female gonad. Vibrio Tapetis infection induced expression of MC-sialec in hemocytes.

저자정보

  • Youngmi Kim Departments of Biochemistry, School of Medicine
  • Mausumi Adhya Departments of Biochemistry, School of Medicine, Department of Physics
  • Kwang-Sik. Choi School of applied marine Science, College of Ocean Science
  • Younghun Yu Department of Physics
  • Moonjae Cho Departments of Biochemistry, School of Medicine, Institute of Medical Science, Cheju National University

참고문헌

자료제공 : 네이버학술정보

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