earticle

영어

IgA Nephropathy and thin basement membrane nephropathy (TBMN) have identical hematuria symptom but different seriousness. In general, the biopsy of the kidney is performed to distinguish between IgAN and TBMN. There are a lot of needs for clinician to have a sensitive and specific biomarker for diseases diagnosis. Glycosylation is highly sensitive to the biological environment changes and considerably affected by disease states. Glycan is known as playing key roles in cancer metastasis and intracellular recognition. Therefore, the examination of glycosylation change in urinary exosome may be a new way for potential biomarkers to differentiate TBMN and IgAN. In this study, we have analyzed 18 individuals exosomes secreted into urine from renal epithelial cells. Samples were consisted of three different groups, mainly TBMN, IgAN patients, and healthy volunteers. Briefly, N-glycans from urinary exosomes were released by PNGase F digestion and then enriched by solid phase extraction using a porous graphitized carbon cartridge. N-glycans were eluted with three different solution (10% ACN, 20% ACN, 40% ACN with 0.05% TFA in H2O) based on the glycan size and polarity. Enriched glycans were analyzed using MALDI-TOF/TOF mass spectrometry and nanoLCchip/QTOF mass spectrometry for qualitative and quantitative profiling, respectively. We found that high mannose glycans are high in abundance in neutral fractions while complex type glycans consisting of [Hex]n=4-6 [HexNAc]n=4-5[Fuc]n=0-1[NeuAc]1 are high in abundance in acidic fractions.