earticle

영어

The glycosaminoglycans (GAGs) from Melibe viridis were extracted and optimized using Multifactorial design. Four factors (Temperature, pH, incubation period, and enzyme ratio) were varied in the optimization experiment. The design used three enzymes namely; Alcalase, Flavourzyme, and Protamex. The optimum results were obtained using Flavourzyme at pH 8.0, incubation temperature of 450C, for 15 hours, and an enzyme ratio of 1.5% relative to sample weight. The extracted Melibe GAGs (prepared at different concentrations) were tested for DPPH scavenging effect, it showed that, it has a comparable activity with standard, the Ascorbic acid. It also showed a reductive capability in a concentration-dependent manner. For the hydroxyl radical scavenging effect, the results showed that the activity is very near with that of the standard too, butylated hydroxyanisole (BHA). From the data obtained in this study, it can be inferred that, Melibe GAGs has a potential in the field of medicine as well as well as they can be used as dietary supplements with specific functional properties