earticle

영어

The polysaccharides extracted from Liparis tesellatus eggs (LEP) and Pronase-digested LEP (LEP-PD) were tested for their anti-inflammatory, anti-cancer, and cytotoxic activities. The anti-inflammatory activities were determined using NO production by macrophage RAW 264.7 cell lines induced by lipopolysaccharide (LPS). Results showed that LEP-PD has an activity on all concentrations (0.01, 0.1, 1.0, 10.0, 100.0, and 200.0 ug/ml), whereas, no activity on all concentrations prepared for LEP. The anti-cancer activities of LEP and LEP-PD were evaluated using Human colon adenocarcinoma (HT-29) and Human stomach adenocarcinoma (AGS) cell lines. Results showed that both the LEP and LEP-PD have anti-carcinogenic effects on these specific cell lines. For the cytotoxic effects of LEP and LEP-PD, RAW 264.7 cell line was also used treated with the polysaccharides. The results showed that LEP and LEP-PD are non-toxic to the cells. From these data obtained, polysaccharides from Liparis tesellatus eggs can be used in the field of medicine upon further purification and elucidation of the structures of the glycans responsible for these promising activities.