earticle

영어

In vivo medical research relies primarily on the common laboratory mouse, who exhibits an inherent similarity to its fellow mammal, the human, yet is much more amenable to experimentation (for ethical, economic, and life cycle -related reasons). Numerous studies have elucidated the genome, proteome, and metabolome of the various mouse models available, but few if any have paid any attention to the mouse glycome. However, glycosylation is one of the most common eukaryotic post-translational modifications, and variations in glycosylation have been linked to cancer and other important human diseases by over fifty years of glycobiological research. Elucidation of the mouse glycome would aid glycomic and glycoproteomic biomarker studies based on the mouse model and advance our understanding of mice as models for humans. We have used retrosynthetic analysis to create a library of all possible mouse serum N-glycan compositions, based on our knowledge of the N-glycan biosynthesis pathway. Highly sensitive nano-LC/MS profiling of mouse serum N-glycans allowed us to annotate the theoretical library with experimental data, while isomer-specific nano-LC/MS/MS provided structural information. The annotated mouse serum N-glycan library was applied to automated data analysis of large sample sets for the purposes of biomarker discovery.