원문정보
초록
영어
Hyaluronan (also called hyaluronic acid or hyaluronate) is an anionic, nonsulfated glycosaminoglycan distributed widely throughout connective, epithelial, and neural tissue. Hyaluronan, a core component of the extracellular matrix, comprises a repeating oligosaccharides of N-acetylglucosamine and D-glucuronic acid. The purpose of this study was to investigate the anti-adipogenic effect of low-molecular weight hyaluronan (LMW-HA, 43,500 Da) on 3T3-L1 preadipocyte cells. The LMW-HA was prepared by enzyme oligosaccharide (Vibrio splindidus BST398) of high-molecular weight hyaluronan (>3x106 Da) produced from Streptococcus zooepidemicus. LMW-HA did not cytotoxicity on 3T3-L1 preadipocyte cells at up to 1000 μg/ml. Treatment at 3T3-L1 cells maintained in adipocyte-induction media with 25, 50, 100, and 200 μg/ml of LMW-HA resulted in significant decrease in intracellular lipid drops by 75.5, 65.4, 58.0, and 53.5 %, respectively, suggesting that LMW-HA has anti-adipogenic effect on 3T3-L1 adipocyte differentiation. Flow cytometry analysis by Annexin V/FITC and PI staining showed that LMW-HA does not affect apoptotic (or necrotic) cell death. However, incubation of cells with LWM-HA 25, 50, 100, and 200 μg/ml) significantly reduced the expression of PPAR-γ by 81, 69, 58, and 56 %, respectively, and also aP2 by 81, 67, 56, and 55 %, respectively, compared to untreated control group. PPAR-γ and aP2 are known as the major adipocyte-specific genes. Therefore, these results suggested that LMW-HA has anti-obesity effect by inhibiting adipocyte differentiation through down-regulation of PPAR-γ and aP2 expression.