earticle

영어

Plant produce complex N-glycans with ß1,2-xylose and core α1,3-fucose residues linked to the conserved core oligosaccharide. Production of heterologous glycoprotein in plants for therapeutic purposes is limited by the presence of plant specific oligosaccharide residues that are considered to arouse immunogenic response in the recipient body. Therefore, efforts for developing humanized N-glycosylation system in plants are required for the large scale production of safe therapeutic glycoproteins. In this regard, we isolated a rice mutant lacking complex N-glycans. However, the mutant showed severe developmental defects such as retarded shoot and root development, failure in tiller formation, and finally resulted in early developmental lethality. It is also shown that the mutant contains low amount of cellulose and high content of lignin. Interestingly, callus induced from the mutant seeds was maintainable and proliferate similarly compared with that of WT despite the size were relatively smaller.