원문정보
초록
영어
Propylene glycol alginate sodium sulfate (PSS) is a kind of sulfated polysaccharide that is derived from seaweed extract sodium alginate through hydrolysis and esterification. PSS is a heparinoid drug, mainly used for the prophylaxis and treatment of ischemic cardiovascular and cerebrovascular diseases in clinical in China. In order to develop and utilize PSS better, a new fluorescent labeling method was established to study the pharmacokinetic parameters of PSS. A rapid and sensitive fluorescent labeling method was developed and validated for microanalysis of marine sulfated polysaccharide PSS in rat plasma. 1, 6-diaminohexane was chosen as the spacer arm to link PSS with FITC. The fluorescent labeled PSS (F-PSS) was identified through its spectroscopic properties. The labeling method showed good linearity, precision, recovery and stability, which suggested that it was sensitive and reliable. The pharmacokinetic results showed that the absolute bioavailability of F-PSS was 8.39% and the other parameters were tested after oral and intravenous administration of F-PSS. The labeling method could be successfully applied to the investigation of the absorption and metabolism of PSS in future. Moreover, the labeling method could be also applied to pharmacokinetic studies of other polysaccharides in biological samples.