원문정보
초록
영어
Cancer seems like unsolvable disease to human beings. Since there are so many types of the disease, it’s harder to figure out full cure for it. We had special interest in curing colorectal cancer. Curing and preventing colorectal cancer, we can use mAb CO17-1A. GA733-2 is a glycoprotein highly expressed in colorectal carcinoma cells. mAb CO17-1A recognizes GA733-2 of carcinoma cells. Since our body is unable to produce mAb CO17-1A, we have to get the mAb from other sources, such as plants or insect cells. In this study, we are going to find out the differences between two insect cells, SF9 and SWT-4, in producing mAb CO17-1A. By performing purification and SDS PAGE, we figured out that there were more mAb productions in SWT-4. Also cells with KDEL showed different types of glycan structures to cells without KDEL. KDEL is a signal peptide that retains proteins in ER. KDEL stands for Lysine, Aspartic Acid, Glutamic Acid, and Leucine. High-performance liquid chromatography (HPLC) verified that cells with KDEL showed high-mannose type glycan while cells without KDEL gave us insect cell specified glycan.