earticle

영어

Trichomonas vaginalis is a flagellated protozoan parasite causing vaginal trichomoniasis in women, and can secrete chemotactic lipid mediator LTB4 in their secretory products. However, signaling mechanisms of tissue inflammatory responses by infection with T. vaginalis are not fully understood. Recently, we have recently demonstrated that human mast cells and neutrophils are activated to produce chemokine IL-8 via LTB4 receptor BLT1 in response to T. vaginalis-derived secretory products (TvSP). Here, we report that O-glycosylation is important in BLT1-mediated IL-8 secretion in human mast cells (HMC-1 cell line) induced by TvSP. Incubation of HMC-1 cells with TvSP resulted in marked increase of O-GlcNacylated proteins and up-regulated IL-8 protein secretion. TvSP-induced IL-8 production and O-glycosylation in HMC-1 cells was inhibited by pretreatment with OGT inhibitor or OGT siRNA. Pretreatment of HMC-1 cells with BLT1 antagonist or BLT1 siRNA strongly abolished the stimulatory effects of TvSP on O-GlcNacylation and IL-8 production. Moreover, TvSP-induced phosphorylation of transcription factors NF-B and CREB for IL-8 production was reduced by pretreatment of HMC-1 cells with OGT inhibitor or OGT siRNA. These results suggest that BLT1-mediated O-glycosylation may play an important role in activation of transcription factors CREB and NF-B for IL-8 production in mast cells stimulated with TvSP, which can contribute to mast cell-mediated tissue inflammatory responses in T. vaginalis-infected lesion during human trichomoniasis.