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PP-26

Detection and Isolation of Dendritic Cells Using Lewis X-functionalized Magnetic Nanoparticles

원문정보

Sara H. Rouhanifard, Ran Xie, Guoxin Zhang, Xiaoming Sun, Xing Chen, Peng Wu

한국당과학회 한국당과학회 학술대회 ACGG 2012 Conference 2012.10 pp.54-55
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초록

영어

Dendritic cells (DCs) are professional antigen-presenting cells that serve as messengers between innate and adaptive immunity. Due to their unique roles, DCs are under active investigations as the target for antigen delivery in vaccination against human immunodeficiency virus (HIV), cancer and autoimmune diseases. However, there’s yet no perfect strategy for a cost-effective, high-selective and time-saving detection and isolation of DCs from a complex cell population. Adhesion molecules such as DC-specific intracellular adhesion molecule-3 grabbing non-integrin (DC- SIGN) on the surface of DCs play a critical role in establishing contact between the activated DCs and the naïve T cells through interactions with intercellular adhesion molecule-3 on T cells. DC-SIGN is a member of the C-type lectin receptors and can recognize antigens bearing mannose-rich or fucosylated glycans, as well as Lewis X (LeX). Here, we present the fabrication of magnetic nanoparticles coated with multivalent LeX glycans using the Cu (I)-catalyzed azide-alkyne cycloaddition. The resulting nanoparticles are selective and biocompatible, serving as a highly efficient tool for DC capture detection and enrichment.

저자정보

  • Sara H. Rouhanifard Department of Biochemistry Albert Einstein College of Medicine, Yeshiva University, 1300 Morris Park Ave, Bronx, NY 10461
  • Ran Xie Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China
  • Guoxin Zhang State Key Laboratory of Chemical Resource Engineering, P.O. Box 98, Beijing University of ChemicalTechnology, Beijing 100029, P. R. China
  • Xiaoming Sun State Key Laboratory of Chemical Resource Engineering, P.O. Box 98, Beijing University of ChemicalTechnology, Beijing 100029, P. R. China
  • Xing Chen Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China
  • Peng Wu Department of Biochemistry Albert Einstein College of Medicine, Yeshiva University, 1300 Morris Park Ave, Bronx, NY 10461

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