원문정보
초록
영어
Embryonic stem cell-preconditioned microenvironment is important for cancer cells properitities by change cell morphology and proliferation. This microenvironment induces cancer cell reprogramming and results in a change in cancer cell properties such as differentiation and migration. The cancer microenvironment affects cancer cell proliferation and growth. However, the mechanism has not been clarified yet. Using the ES-preconditioned 3-D microenvironment model, we provide evidence showing that the ES microenvironment inhibits proliferation and reduces oncogenic gene expression. But ES microenvironment has no effect on telomerase activity, cell viability, cellular senescence, and methylation on Oct4 promoter region. Furthermore, methylation of Nanog was increase on ES-preconditioned microenvironment and supports results that no difference on RNA expression levels. Taken together, these results demonstrated that in the ES-preconditioned 3-D microenvironment is a crucial role for cancer cell proliferation not senescence.
목차
INTRODUCTION
MATERIALS AND METHODS
Cell Culture
Feeder Preparation and Embryonic Stem (ES) Cell Culture
Preparation of 3D-Conditioned Matrix and Reprogramming Induction
Cell Viability Test
Bisulfite Sequencing
RNA Extraction and Real Time RT-PCR
3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfonyl)-2H-tetrazolium Assay
Cell Cycle Analysis
Telomerase Activity Assay
Cellular Aging Assay
RESULTS
The ES-Preconditioned Microenvironment Induces MorphologicalChanges
The ES-Preconditioned Microenvironment Induces Lossof Cancer Cell Properities
The ES-Preconditioned Microenvironment Not Induces Cellular Senescence
ES-Preconditioned Microenvironment Suppress OncogenicGene Expression
DISCUSSION
Acknowledgements
REFERENCES